Group of immunology

 

 

Group of Immunology was established in 2007 as a successor to the Group for immunochemistry. Changing the name of a group emerged as a result of broadening the scope of research, from immunochemical properties of immunoglobulins, immune complexes and lipoproteins to the molecular and cellular mechanisms of immunoregulation.

Until 2007, the research, in the Group of immunology, was focused on structure-based analysis of immunoglobulins, the humoral branch of immune response, in body fluids of humans and animals, underlying their physiological and pathological conditions. The results of the studies of molecular mechanisms of malignant alterations in multiple myeloma have provided the following information: structural characteristics of serum and urinary monoclonal immunoglobulins (paraprotein) in human malignant monoclonal gammopathies; activity of two inherited immunoglobulin gene loci; and composition of cell receptors expressed on peripheral blood lymphocyte in multiple myeloma. Over this period a notable part of the work was related to the problem of reactivity and specificity of rheumatoid factors, auto anti-immunoglobulin antibodies present in the serum of patients with rheumatoid arthritis. Another major area of investigation was defining the molecular characteristics of immunoglobulins in the sense of local mucosal immune system response. The most important goal of this area of research was to analyse the molecular characteristics of immunoglobulins of oral fluid, gingival crevicular fluid and saliva in periodontal disease.

Since 2007, due to opening new research areas, the members of the group have introduced, in coordination with other institutes and faculties of the University of Belgrade, a number of new methods and techniques: cell culture, cutting edge microscopic techniques, complex physicochemical analyses, and novel techniques to test nucleic acids. The current research is mainly focused on studies of the immunomodulatory function of immune complex, mesenchymal stem cells, nutrients, and hormones. Additionally, the group members participated in cell signalling research, pertaining to the mechanisms of immunoregulation, through which proinflammatory cytokines regulate growth and functions of hematopoietic and non-haematopoietic cell of significance for effective immune response. In reference to applied research the group members have analysed anti-inflammatory role of conventional and newly synthesized steroidal and non-steroidal drugs administered in the form of free molecules or in the form of system for prolonged release based on erythrocyte membranes. The group has carried out studies on body fluids’ proteins and lipoproteins with the aim of using them as biomarkers of inflammatory diseases in humans and animals. In conclusion, both basic and applied research performed in the group led to the development of a significant number of immunochemical assays.

The Group of Immunology is headed by dr. Vesna Ilić, and the associates are: dr. Dragana Marković, dr. Sonja Mojsilović, dr. Ivana Drvenica, dr. Marijana Kovačić, dr. Irina Maslovarić and dr. Ana Stančić.

 

Group members

 

Dr. Vesna Ilić, Principal Research Fellow (head of group)

 

Dr. Dragana Marković, Senior Research Associate

 

Dr. Ivana Drvenica, Senior Research Associate

 

Dr. Sonja Mojsilović, Research Associate

Dr. Irina Maslovarić, Research Associate
 
 

Dr. Marijana Kovačić, Research Associate

 

Dr. Ana Stančić, Research Assistant

Educational activity

 

In the period of 2008 - 2017. members of Group of immunology, IMR participated in 15 PhD thesis, 3 master thesis and 5 graduation papers at the Faculty of Biology, Faculty of Technology and Metallurgy, Faculty of Medicine and Faculty of Veterinary Medicine, University of Belgrade.

Projects

Hemoglobin-based spectroscopy and nonlinear imaging of erythrocytes and their membranes as emerging diagnostic tool (HEMMAGINERO) (2021-2022) PROMIS

Hemoglobin-based spectroscopy and nonlinear imaging of erythrocytes and their membranes as emerging diagnostic tool (HEMMAGINERO). Program for excellent projects of young researchers in Republic of Serbia (PROMIS)  funded by The Science Fund of the Republic of Serbia. Project leader: Dr Aleksandar krmpot, Institute for Physics, University of Belgrade. Dr Ivana Drvenica, project participant.

Regenerative and modulatory potential of adult stem cells - OI175062 (2011-2019) (National project)

Funded by the Serbian Ministry of Education, Science and Technology Development (PI dr. Diana Bugarski)

Effect of resistance training on the functional characteristics of peripheral blood cells (2011-2012)(National project)

Funded by the Serbian Ministry of Youth and Sports. Coordinators: dr Vesna Ilić, IMR, University of Belgrade; dr Irina Maslovarić, IMR, weightlifting club "Red Star" and the Serbian Weightlifting Federation; dr. Sanja Mazić, Serbian Institute of Sport and Sports Medicine, Faculty of Medicine, University of Belgrade

Publications

Insight into the Biological Activity of Hennosides-Glucosides Isolated from Lawsonia inermis (henna): Could They Be Regarded as Active Constituents Instead (2021)

Maslovarić I, Ilić V, Drvenica I, Stančić A, Mojsilović S, Kukolj T, Bugarski D, Saso L, Nicoletti M. (2021) Insight into the Biological Activity of Hennosides-Glucosides Isolated from Lawsonia inermis (henna): Could They Be Regarded as Active Constituents Instead. Plants (Basel). 10(2):237

Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes (2020)

Kovačić M, Natalija Fratrić, Aleksandra Arsić A, Mojsilović Sl, Drvenica I, Marković D, Maslovarić I, Grujić Milanović J, Ivanov M, Ilić V. (2020) Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes. Research in Veterinary Science, 63-74. 

Effects of oral supplementation with organically modified clinoptilolite during prepartum period on colostrum quality in primiparous dairy cows (2020)

Stojić M, Ilić V, Kovačić M, Gvozdić D, Stajković S, Vejnović B, Savić O, Fratrić N. (2020) Effects of oral supplementation with organically modified clinoptilolite during prepartum period on colostrum quality in primiparous dairy cows. Journal of Dairy Research, 87(4): 429-435

Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro (2020)

Stančić AZ, Drvenica IT, Obradović HN, Bugarski BM, Ilić VLj, Bugarski DS. (2020) Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro. International Journal of Biological Macromolecules, 144:909-920.

Extracellular xenogeneic hemoglobin suppresses myogenic differentiation capacity of C2C12 myoblasts (2020)

Stančić AZ, Drvenica IT, Bugarski BM, Ilić VLj, Bugarski DS. (2020) Extracellular xenogeneic hemoglobin suppresses myogenic differentiation capacity of C2C12 myoblasts, Archives of Biological Sciences, 72(3):379-391.

Maltose-mediated long-term stabilization of freeze- and spray- dried forms of bovine and porcine hemoglobin (2019)

Drvenica I, Stančić A, Kalušević A, Marković S, Dragišić Maksimović J, Nedović VA, Bugarski BM, Ilić VLj. (2019) Maltose-mediated long-term stabilization of freeze- and spray- dried forms of bovine and porcine hemoglobin. Journal of the Serbian Chemical Society, 84(10): 1105-1117.

Erythrocytes Membranes: Unique Constituent of Biological/Hybrid Drug Delivery Systems (2019)

Drvenica I, Stančić A, Bugarski B, Pajic-Lijaković I, Maslovarić I, Ilić V. (2019) Erythrocytes Membranes: Unique Constituent of Biological/Hybrid Drug Delivery Systems, In: Erythrocytes: Structure, Functions and Clinical Aspects by Katy Jorissen (Editor), Series: Recent Advances in Hematology Research, Nova Science Publishers, Inc.

Platelet-poor plasma of athletes is a potent inducer of myogenic differentiation of C2C12 myoblasts (2019)

Maslovarić I, Ilić VLj, Stančić AZ, Santibanez JF, Trivanović DI, Drvenica IT, Krstić JS, Mojsilović SB, Okić Djordjević IN, Bugarski DS. (2019) Platelet-poor plasma of athletes is a potent inducer of myogenic differentiation of C2C12 myoblasts, Veterinarski glasnik, https://doi.org/10.2298/VETGL190414019M.

Transfusion-transmissible infections among Serbian blood donors: declining trends over the period 2005-2017 (2019)

Vučetić D, Jovičić M, Maslovarić I, Bogdanović S, Antić A, Stanojković Z, Filimonović G, Ilić V. (2019) Transfusion-transmissible infections among Serbian blood donors: declining trends over the period 2005-2017. Blood Transfusion, 17(5):336-346.

Vaccination and autoimmunity: influenza vaccination and association with multiple sclerosis (2018)

Maslovarić I, Stojković A, Kosanović D, Marković D, Ilić V, Jovanova-Nešić K. (2018) Vaccination and autoimmunity: influenza vaccination and association with multiple sclerosis. Vojnosanitetski pregled, 75(7): 721–729.

 

Flow cytometry analysis of platelets’ populations is useful in monitoring storage lesions in pooled buffy-coat platelet concentrates (2018)

Vučetić D, Ilić V, Vojvodić D, Subota V, Todorovi M, Balint B. (2018) Flow cytometry analysis of platelets’ populations is useful in monitoring storage lesions in pooled buffy-coat platelet concentrates, Blood Transfusion 16(1): 83-92.

 

Investigation of Metabolic Properties and Effects of 17β-carboxamide Glucocorticoids on Human Peripheral Blood Leukocyte (2018)

Dobričić V, Drvenica I, Stančić A, Mihailović M, O Čudina O, Bugarski D,  Ilić V. (2018) Investigation of Metabolic Properties and Effects of 17β-carboxamide Glucocorticoids on Human Peripheral Blood Leukocyte. Archiv der Pharmazie 351(5):e1700371

Mapping of hemoglobin in erythrocytes and erythrocyte ghosts using two photon excitation fluorescence microscopy. (2017)

Bukara K, Jovanić S, Drvenica IT, Stančić A, Ilić V, Rabasović MD, Pantelić D, Jelenković B, Bugarski B, Krmpot AJ. Mapping of hemoglobin in erythrocytes and erythrocyte ghosts using two photon excitation fluorescence microscopy. (2017) Journal of Biomedical Optics, 22(2):26003. doi: 10.1117/1.JBO.22.2.026003.

Serum proteins and lipids in mild form of calf bronchopneumonia: Candidates for reliable biomarkers Acta Veterinaria-Beograd (2017)

Kovačić M, Marković D, Maslovarić I, Obrenović S, Grujić-Milanović J, Arsić A, Milanović Z, Savić O, Fratrić N, Ilić V. (2017) Serum proteins and lipids in mild form of calf bronchopneumonia: Candidates for reliable biomarkers Acta Veterinaria-Beograd, 67(2), DOI:10.1515/acve-2017-0018.

Effects of selenium and thyroid hormone deficiency on peritoneal macrophages adhesion and occurrence of naturally occurring IgM antibodies in juvenile rats (2017)

Bjelica J, Jovanović IB, Ilić V, Valčić O, Kirovski D, Gvozdić D, Milanović S. (2017) Effects of selenium and thyroid hormone deficiency on peritoneal macrophages adhesion and occurrence of naturally occurring IgM antibodies in juvenile rats. Acta Veterinaria-Beograd, in press

Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer (2017)

Stojić M, FratrićN, KovačićM, Ilić V, Gvozdić D, Savić O, Đoković R, Valčić O. (2017) Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer. Acta Veterinaria-Beograd, in press

Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation. (2016)

Buač M, Mojsilović S, Mišić D, Vuković D, Savić O, Valčić O, Marković D, Gvozdić D, Ilić V, Fratrić N. (2016) Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation. Research in Veterinary Science, 106: 135-142.

Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate. (2016)

Drvenica I, Bukara K, Ilić V, Mišić D, Vasic B, Gajic R, Djordjević V, Veljović Dj, Belić A, Bugarski B. Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate. (2016) Biotechnology Progress, 32: 1046-1055.

Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts (2016)

Bukara K, Drvenica I, Ilić V, Stančić A, Mišić D, Vasić B, Gajić R, Vučetić D, Kiekens F, Bugarski B. (2016) Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts. Journal of Biotechnology 240: 14-22.

Flow cytometry analysis of platelets’ populations is useful in monitoring storage lesions in pooled buffy-coat platelet concentrates (2016)

Vučetić D, Ilić V, Vojvodić D, Subota V, Todorović M, Balint B. (2016) Flow cytometry analysis of platelets’ populations is useful in monitoring storage lesions in pooled buffy-coat platelet concentrates. Blood Transfusion, DOI 10.2450/2016.0193-16.

Seroprevalence of IgG antibodies against Borrelia burgdorferi in dogs in Belgrade area, Serbia (2015)

Obrenović S, Ristanović E, Čekanac R, Radulović Ž, Ilić V. (2015) Seroprevalence of IgG antibodies against Borrelia burgdorferi in dogs in Belgrade area, Serbia. Acta Veterinaria-Beograd 65: 99-110.

Blood donors' transfusion-transmissible infection positivity - the Serbian Military Medical Academy experience. (2015)

Vučetić D, Kecman G, Ilić V, Balint B. Blood donors' transfusion-transmissible infection positivity - the Serbian Military Medical Academy experience. (2015) Blood Transfusion, 13: 569-575.

Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery (2015)

Kostić IT, Ilić VL, Bukara KM, Mojsilović SB, Đurić Z, Draškovič P, Bugarski BM. (2015) Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery. Hemijska industrija Hemijska industrija 69: 67–76

Erythrocyte membranes from slaughterhouse blood as potential drug vehicles: Isolation by gradual hypotonic hemolysis and biochemical and morphological characterization (2014)

Kostić IT, Ilić VLj, Ðorđević VB, Bukara KM, Mojsilović SB, Nedović VA, Bugarski DS, Veljović ÐN, Mišić DM, Bugarski BM (2014) Erythrocyte membranes from slaughterhouse blood as potential drug vehicles: Isolation by gradual hypotonic hemolysis and biochemical and morphological characterization. Colloids and Surfaces B: Biointerfaces, 122, 250-259.

 

Specific binding of paraprotein to platelet receptors as a cause of platelet dysfunction in monoclonal gammopathies (2013)

Djunic I, Elezovic I, Vucic M, Srdic-Rajic T, Konic-Ristic A, Ilic V, Milic N, Bila J, Suvajdzic-Vukovic N, Virijevic M, Antic D, Vidovic A, Tomin D. (2013) Specific binding of paraprotein to platelet receptors as a cause of platelet dysfunction in monoclonal gammopathies. Acta Haematologica. 130: 101-107.

Immunomodulatory capacity of human mesenchymal stem cells isolated from adipose tissue, dental pulp, peripheral blood and umbilical cord Wharton’s Jelly (2013)

Trivanović D, Mojsilović S, Ilić V, Krstić J, Jauković A, Okić-Đorđević I, Santibanez JF, Jovčić G, Bugarski D. (2013) Immunomodulatory capacity of human mesenchymal stem cells isolated from adipose tissue, dental pulp, peripheral blood and umbilical cord Wharton’s Jelly. Central European Journal of Immunology 38: 421-429.

Evidence that calf bronchopneumonia may be accompanied by increased sialylation of circulating immune complexes` IgG.(2012)

Fratrić N, Gvozdić D, Vuković D, Savić O, Buač M, Ilić V. (2012) Evidence that calf bronchopneumonia may be accompanied by increased sialylation of circulating immune complexes` IgG. Veterinary immunology and immunopathology, 150: 161– 168.

Serum amyloid A isoforms in serum and milk from cows with Staphylococcus aureus subclinical mastitis (2012)

Kovačević-Filipović M, Ilić V, Vujčić Z, Dojnov B, Stevanov-Pavlović M, Mijačević Z, Božić T. (2012) Serum amyloid A isoforms in serum and milk from cows with Staphylococcus aureus subclinical mastitis. Veterinary immunology and immunopathology, 145: 120-128.

Isolation of hemoglobin from bovine erythrocytes by controlled hemolysis in the membrane bioreactor (2012)

Stojanović R, Ilić V, Manojlović V, Bugarski D, Dević M, Bugarski B. (2012) Isolation of hemoglobin from bovine erythrocytes by controlled hemolysis in the membrane bioreactor. Applied Biochemistry and Biotechnology, 166: 1491-1506.

Anion exchange chromatographic distribution of human monoclonal immunoglobulin G is determined by heavy chain subclass and level of sialic acid expression (2012)

Ilić V, Mojsilović S, Marković D, Maslovarić I, Milošević-Jovčić N. (2012) Anion exchange chromatographic distribution of human monoclonal immunoglobulin G is determined by heavy chain subclass and level of sialic acid expression. Hemijska industrija, 66: 531–540.

Thesis

Ana Stančić (2020) PhD thesis

Ana Stančić (2020) Effect of extracellular hemoglobin of xenogeneic origin on functional characteristics of mesenchymal cells in vitro. Faculty of Biology, University of Belgrade.

Marijana Kovačić (2018) - PhD thesis

Marijana Kovačić(2018) Structural and functional properties of immune complexes in calves with bronchopneumonia. Faculty of Chemistry, University of Belgrade

Irina Maslovarić (2016) PhD thesis

Role of inactivated influenza vaccine in regulation of autoimmune processes in experimental autoimmune encephalomyelitis. Faculty of Biology, University of Belgrade

Sonja Mojsilović (2016) PhD thesis

Influence of pro‐ and anti‐inflammatory factors on production of extracellular matrix proteases uPA and MMP9 in mouse macrophages. Faculty of Biology, University of Belgrade

Ivana Drvenica (nee Kostić) (2015) PhD thesis

Ivana Kostić (2015) ​Preserved erythrocyte membranes produced from slaughterhouse blood as systems for prolonged delivery of active substances. Faculty of Technology and Metallurgy, University of Belgrade

Dragana Marković (2010) PhD thesis

Molecular characteristics, interactions, and pathogenic potential of rheumatoid factor type anti-IgG autoantibodies in rheumatoid arthritis. Faculty of Biology, University of Belgrade

Vesna Ilić (2005) PhD thesis

Immunoglobulin G of peripheral blood B-lymphocytes in multiple myeloma: molecular characteristics and possible influence to the immunoregulation disorders. ​2005, Faculty of Biology, University of Belgrade